Raman spectroscopy is a non-destructive and non-invasive analytical technique that can be used for the analysis of biological samples. This technique is based on the inelastic scattering of light, which can provide information about the chemical and structural properties of the sample. Due to its non-destructive nature, Raman spectroscopy can be used to study living systems, such as cells and tissues, without altering their properties.
Raman spectroscopy has been used for the analysis of a wide range of biological samples, including cells, tissues, proteins, and DNA. This technique can be used to study the chemical composition of biological samples, to identify specific biomolecules, and to study the interactions between biomolecules.
Raman spectroscopy is particularly useful for studying the biochemical changes that occur during disease development, such as cancer. It can also be used to study the effects of drugs on biological samples, which can provide insight into how drugs work at the molecular level.
However, Raman spectroscopy also has some limitations when it comes to the analysis of biological samples. One of the main limitations is the low sensitivity of the technique, which can make it difficult to detect weak Raman signals from biological samples. Additionally, Raman spectroscopy can be affected by fluorescence, which is a type of scattered light that can interfere with the measurement. Fluorescence is particularly problematic when studying biological samples because they naturally fluoresce.
In conclusion, Raman spectroscopy is a powerful analytical technique that can be used for non-invasive analysis of biological samples. It can provide valuable information about the chemical and structural properties of biological samples and can be used to study the effects of drugs on biological samples. However, it also has some limitations, such as low sensitivity and fluorescence interference.